Effect of age, potassium depletion and denervation on specific displaceable [3H]ouabain binding in rat skeletal muscle in vivo

AUTOR(ES)
RESUMO

1. Following intraperitoneal injection of [3H]ouabain in rats, the isotope is rapidly distributed in blood plasma available for binding to the Na-K-ATPase in the plasma membranes of most tissues. In skeletal muscle tissue excised and washed 4 × 30 min in ice-cold buffer, 95% of the 3H activity retained was shown to be [3H]ouabain using a specific binding assay.

Documentos Relacionados