Effect of NAD+ on Malate Oxidation in Intact Plant Mitochondria 1

AUTOR(ES)

Tobin, Alyson

RESUMO

Potato tuber mitochondria oxidizing malate respond to NAD+ addition with increased oxidation rates, whereas mung bean hypocotyl mitochondria do not. This is traced to a low endogenous content of NAD+ in potato mitochondria, which prove to take up added NAD+. This mechanism concentrates NAD+ in the matrix space. Analyses for oxaloacetate and pyruvate (with pyruvate dehydrogenase blocked) are consistent with regulation of malate oxidation by the internal NAD+/NADH ratio.

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