Effect of the Fv-1 locus on the titration of murine leukemia viruses.
AUTOR(ES)
Jolicoeur, P
RESUMO
Titration of N- and B-tropic murine leukemia viruses on sensitive and resistant cell lines has been studied by direct XC plaque assay and infective center assay. The titration of cloned B-tropic virus by infective center assay on BALB/3T3 (Fv-1b/b) and NIH/3T3 (Fv-1n/n) cells gave one-hit patterns, with 100-fold less infected NIH/3T3 cells than BALB/3T3 cells. The titration of B-tropic virus on DBA/2 cells (Fv-1n/n) was also a one-hit. The titration of a one-hit curve, and there were about 100-fold less infected BALB/3T3 cells than NIH/3T3 cells. Comparable results were obtained by titrating the cloned N-tropic virus on congenic SIM (Fv-1n/n) and SIM.R (Fv-1b/b) cells or the Gross N-tropic virus on BALB/3T3 cells. Therefore, our data indicate that the multiple-hit phenomenon described previously may not be an essential part of the Fv-1 gene restriction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355768Documentos Relacionados
- Macromolecular requirements for abrogation of Fv-1 restriction by murine leukemia viruses.
- Effect of Fv-1 gene product on proviral DNA formation and integration in cells infected with murine leukemia viruses.
- Physical mapping of the Fv-1 tropism host range determinant of BALB/c murine leukemia viruses.
- Genetic evidence for a product of the Fv-1 locus that transfers resistance to mouse leukemia viruses.
- Transfection of Fv-1 permissive and restrictive mouse cells with integrated DNA of murine leukemia viruses.