Effects caused by the spinal administration of ketamine S (+) 5% with no preservatives, using a single puncture, and located on the spinal cord and meninges in rabbits
AUTOR(ES)
Lima Filho, José Admirço, Fin, Natalia Castro, Valerini, Felipe Gilberto, Machado, Vania Maria, Marques, Mariangela Ester, Miot, Hélio, Lima, Lais Helena Navarro e, Ganen, Eliana Marisa
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2014-07
RESUMO
PURPOSE:To evaluate the effect of ketamine S (+) 5% with no preservatives and administered as a subarachnoid single puncture on the spinal cord and meninges of rabbits.METHODS:Twenty young adult female rabbits, each weighing 3500-5000 g and having a spine length between 34 and 38 cm, were divided by lot into two groups (G): 0.9% saline in G1 and ketamine S (+) 5% in G2, by volume of 5 μg per cm column (0.18 mL). After intravenous anaesthesia with ketamine and xylazine, the subarachnoid space was punctured at S1-S2 under ultrasound guidance, and a random solution was injected. The animals remained in captivity for 21 days under medical observation and were sacrificed by decapitation. The lumbosacral spinal cord portion was removed for immunohistochemistry to assess the glial fibrillary acidic protein (GFAP), and histology was assessed using hematoxylin and eosin (HE) stain.RESULTS: No histological lesions were found in the nervous tissue (roots and cord) or meninges in either group.CONCLUSION:The ketamine S (+) 5% unpreserved triggered no neurological or histological lesions in the spinal cord or meninges of rabbits.
Documentos Relacionados
- The effects of cyproheptadine on locomotion and on spasticity in patients with spinal cord injuries.
- Effects of bathing on Pseudomonas and Klebsiella colonization in patients with spinal cord injuries.
- Superficial cerebral and spinal haemosiderosis caused by secondary tethered cord syndrome after resection of a spinal lymphoma
- THE EFFECT OF MONOSIALOGANGLYOSIDE (GM-1) ADMINISTRATION IN SPINAL CORD INJURY
- Effects of alpha-melanocyte stimulating hormone on fever caused by endotoxin in rabbits.