Effects of dilute HCl on yeast tRNAPhe and E. coli tRNAfMet1

AUTOR(ES)

Ladner, Jane E.

RESUMO

HCl treatment of yeast tRNAPhe under conditions generally used for excision of `Y' base results in structure and conformation changes as monitored by line widths in the PMR spectra at 220 MHz and by optical rotation. Like exposure of E. coli tRNAfMet1 causes similar changes in the PMR spectra and optical rotation although no residues are eliminated. Electrophoresis in polyacrylamide gels provides evidence for aggregation in HCl-treated tRNAfMet1. One must thus consider a general effect of HCl exposure as well as possible residue removal in assessing induced structural and conformation changes in tRNA.

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