Effects of Environmental Factors on the Survival of Airborne T-3 Coliphage
AUTOR(ES)
Ehrlich, Richard
RESUMO
Experiments were conducted to determine the effects of storage temperatures, relative humidity, and additives on the survival of aerosolized Escherichia coli phage T-3. The aerosol stability of the coliphage, calculated as per cent recovery, was not affected by storage at 10 or -70 C for up to 4 months. However, an increase in aerosol decay rate of coliphage stored at 10 C was observed. The effect of humidities ranging from 20 to 90% relative humidity was studied, and it was observed that humidities lower than 70% relative humidity significantly reduce the survival of airborne coliphage. The effect of various compounds on the aerosol decay rate of T-3 coliphage was studied at 50 and 85% relative humidity. Addition of dextrose in 0.1 M concentrations to the disseminating fluid significantly reduced aerosol decay rate at 50% relative humidity without affecting the decay at 85%. Addition of spermine, spermidine-phosphate, thiourea, galacturonic acid, and glucosaminic acid, individually or in combination, had no effect on aerosol decay rates. The use of deuterium oxide as the suspending fluid for dissemination had no effect on aerosol stability of the coliphage.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1058163Documentos Relacionados
- A Slit Sampler for Collecting T-3 Bacteriophage and Venezuelan Equine Encephalomyelitis Virus: I. Studies with T-3 Bacteriophage
- Effects of Atmospheric Humidity and Temperature on the Survival of Airborne Flavobacterium
- Survival of T3 Coliphage in Varied Extracellular Environments. I. Viability of the Coliphage During Storage and in Aerosols1
- Enhanced Recovery of Airborne T3 Coliphage and Pasteurella pestis Bacteriophage by Means of a Presampling Humidification Technique
- Reaction of Airborne Rhizobium meliloti to Some Environmental Factors