Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation
AUTOR(ES)
Machado, Natasha, Duailibi, Silvio Eduardo, Santos, Jennifer Adriane dos, Penna, Vanessa, Ferreira, Lydia Masako, Duailibi, Monica Talarico
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2014-10
RESUMO
PURPOSE: To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation. METHODS: Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity. RESULTS: A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate. CONCLUSION: Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation.
Documentos Relacionados
- Transplantation of human immature dental pulp stem cell in dogs with chronic spinal cord injury
- Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
- Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury
- Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
- Dose-dependent effects of neem crude extract on human dental pulp cell and murine osteoblast viability and mineralization