Effects of Iron and Oxygen on Chlorophyll Biosynthesis 1: II. OBSERVATIONS ON THE BIOSYNTHETIC PATHWAY IN ISOLATED ETIOCHLOROPLASTS

AUTOR(ES)

Chereskin, Barbara M.

RESUMO

The conversion of l-glutamate to δ-aminolevulinate, in preparations of cucumber etiochloroplasts incubated in vitro, was inhibited by protoheme IX and Mg-protoporphyrin IX. Mg-protoporphyrin IX was destroyed in the presence of air and plastids; this breakdown was accelerated by S-adenosyl methionine. Mg-protoporphyrin IX was also converted to protochlorophyllide in vitro. This conversion exhibited an absolute requirement for atmospheric oxygen and was strongly stimulated by S-adenosyl methionine and by darkness.

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