Effects of Osmotic Shock on Some Membrane-regulated Events of Oat Coleoptile Cells 1

AUTOR(ES)

Rubinstein, Bernard

RESUMO

Oat coleoptile sections (Avena sativa L. cv. “Garry”) were osmotically shocked with 0.5 m mannitol followed by 1 mm Na-phosphate (pH 6.4) at 4 C. This treatment reduced uptake of α-aminoisobutyric acid, 3-o-methyl glucose, and leucine by 75 to 90% but inhibited 36Cl− uptake only 30%. Some recovery was observed 1 to 3 hours later. Respiration rates were unaffected by osmotic shock and protein synthesis was reduced 11%.

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