Effects on Bacillus subtilis of a conditional lethal mutation in the essential GTP-binding protein Obg.
AUTOR(ES)
Kok, J
RESUMO
The obg gene is part of the spo0B sporulation operon and codes for a GTP-binding protein which is essential for growth. A temperature-sensitive mutant in the obg gene was isolated and found to be the result of two closely linked missense mutations in the amino domain of Obg. Temperature shift experiments revealed that the mutant was able to continue cell division for 2 to 3 generations at the nonpermissive temperature. Such experiments carried out during sporulation showed that Obg was necessary for the transition from vegetative growth to stage 0 or stage II of sporulation, but sporulation subsequent to these stages was unaffected at the nonpermissive temperature. Spores of the temperature-sensitive mutant germinated normally at the nonpermissive temperature but failed to outgrow. The primary consequence of the obg mutation may be an alteration in initiation of chromosome replication.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=197102Documentos Relacionados
- Biochemical characterization of the essential GTP-binding protein Obg of Bacillus subtilis.
- Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis.
- YsxC, a Putative GTP-Binding Protein Essential for Growth of Bacillus subtilis 168
- The SpoIIAA protein of Bacillus subtilis has GTP-binding properties.
- The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein.