Efficient transfection of the archaebacterium Halobacterium halobium.
AUTOR(ES)
Cline, S W
RESUMO
We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA. Between 0.5 and 1% of spheroplasts were transfected per microgram of luminal diameter H DNA. Under our conditions, survival and regeneration of H. halobium spheroplasts were also quite efficient, suggesting that this method will be useful for introducing other DNAs into these bacteria.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211943Documentos Relacionados
- Polyadenylated RNA isolated from the archaebacterium Halobacterium halobium.
- Putative promoter region of rRNA operon from archaebacterium Halobacterium halobium.
- Complex promoter pattern of the single ribosomal RNA operon of an archaebacterium Halobacterium halobium.
- The number, physical organization and transcription of ribosomal RNA cistrons in an archaebacterium: Halobacterium halobium.
- Chemosensory responses of Halobacterium halobium.