Elaboration of Bacillus anthracis Antigens in a New, Defined Culture Medium
AUTOR(ES)
Ristroph, Joseph D.
RESUMO
Improved culture conditions and a new, completely synthetic medium (R medium) were developed to facilitate the production of Bacillus anthracis holotoxin antigens. Levels of these antigens up to fivefold greater than the highest previously reported values were recovered with the described system. Cultures of Sterne, V770-NP1-R, and Vollum 1B strains of B. anthracis were monitored for growth, pH change, glucose utilization, supernatant protein concentration, lethal toxin activity, and protease activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=347970Documentos Relacionados
- STUDIES ON IMMUNITY IN ANTHRAX IV. : Factors Influencing Elaboration of the Protective Antigen of Bacillus anthracis in Chemically Defined Media
- A New Culture Medium for the Tubercle Bacillus
- Selective medium for Bacillus anthracis.
- Continuous Culture of Ruminal Microorganisms in Chemically Defined Medium1: II. Culture Medium Studies
- Microcycle Sporogenesis of Bacillus cereus in a Chemically Defined Medium