Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.

AUTOR(ES)

Toneguzzo, F

RESUMO

The technique of DNA transfer by electroporation was investigated in an effort to evaluate its utility for the identification of developmentally controlled regulatory sequences. Transient and stable gene expression was detected in a variety of lymphoid cell lines subjected to electroporation. No correlation existed between the levels of chloramphenicol acetyltransferase (acetyl-CoA; chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28) expression and stable transfection frequency. In all lymphoid cell lines tested, the simian virus 40 early region was a better promoter than was the Rous sarcoma virus long terminal repeat.

Documentos Relacionados

• Electric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells.
• Stable expression of selectable genes introduced into human hematopoietic stem cells by electric field-mediated DNA transfer.
• Transient expression of the chicken lysozyme gene after transfer into human cells.
• Recombination events after transient infection and stable integration of DNA into mouse cells.
• Versatile cloning vectors for transient gene expression and direct gene transfer in plant cells.