Electrogenic Sucrose Transport in Developing Soybean Cotyledons 12
AUTOR(ES)
Lichtner, Francis T.
RESUMO
Addition of sucrose to a solution bathing an excised developing soybean cotyledon causes a transient depolarization of the membrane potential, as measured using standard electrophysiological techniques. The magnitude of the depolarization is dependent on the concentration of both sucrose and protons in a manner which suggests carrier mediation; this process has an apparent Km for sucrose of about 10 millimolar. Agents interfering with the generation or maintenance of a proton electrochemical gradient eliminate these depolarizations. Electrogenic sugar transport is sensitive to sulfhydryl-modifying reagents; their effect appears to be through a direct interaction with the carrier protein and/or with the process establishing the proton electrochemical gradient across the plasma membrane. p-Chloromercuribenzene sulfonate appears to be a selective inhibitor of the carrier-mediated process itself.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=425789Documentos Relacionados
- Sucrose Uptake by Developing Soybean Cotyledons 1
- Identification of Membrane Protein Associated with Sucrose Transport Into Cells of Developing Soybean Cotyledons 1
- Involvement of Phospholipids in Triglyceride Biosynthesis by Developing Soybean Cotyledons 12
- Asparagine amide metabolism in developing cotyledons of soybean
- Studies on Lipid Synthesis and Degradation in Developing Soybean Cotyledons 1
