Electron microscopic morphometric studies on the resting and secreting nasal (salt) glands of the domestic duck. I. Standardisation of the fixation procedure.
AUTOR(ES)
Butt, M M
RESUMO
In order to perform morphometric analysis of the nasal (salt) glands of domestic duck it was necessary to standardise the regime of fixation and processing for this tissue. This paper reports the results of these experiments in which the nasal glands of nine groups of ducks were perfused by fixatives containing various concentrations of glutaraldehyde; each group included three secreting ducks and three non-secreting control ducks. Fixative osmolality ranged from 300-825 m-osmol. The resulting effects on the electron microscopical appearances of mitochondria and intercellular spaces were assessed. The fixative finally selected as most satisfactory for both control and secreting tissue contained 3.5% glutaraldehyde and 3% dextran in sodium cacodylate buffer, giving an osmolality range of 580-650 m-osmol.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1166404Documentos Relacionados
- Cholinesterase activity in the supra-orbital salt secreting gland of the duck.
- The nasal glands of birds: A histological and histochemical study of the inactive gland in the domestic duck
- The number, distribution and density of muscle spindles in two wing muscles of the domestic duck.
- Studies on Effect of Certain Quinones: I. Electron Transport, Photophosphorylation, and CO2 Fixation in Isolated Chloroplasts 1
- The ultrastructure of the epithelium of the ducts of the Harderian and lacrimal glands of the turkey, fowl and duck.