EletroquÃmica acoplada à ressonÃncia de plÃsmons de superfÃcie no estudo de adsorÃÃo de proteÃna em filme fino

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Biosensor is a device in which the biological source material, such as enzyme, organelle, animal or vegetable tissue, microorganism, antigen or antibody, nucleic acids, lectina, among others is immobilized close to an adequate transducer. Therefore, the electrode/biomolecule/ solution interface characterization consists in one of the main stages for biosensors development. The goal of this work was to study the gold/lectina interface using the Electrochemistry Impedance Spectroscopy (EIS) and Surface PlÃsmons Resonance (SPR) techniques as transduction means for the confection of a future biosensor. Lectinas belong to a proteins group with specificity to carbohydrates. This characteristic enlarged the dissertation focus for the Concanavalina A protein and the glycogen carbohydrate interaction. In this work it was set up a SPR s system coupled or not to an electrochemistry system, with the intention of monitoring and characterizing the protein adsorption in different potentials and its interaction with carbohydrates. With base in the obtained results, the protein in the disabled configuration adsorbed more than the activated form, however, the activated configuration better recognized the carbohydrate. Besides, it was verified that the protein, both configurations, adsorbs more easily in more positive potentials, due to a larger charges contrast between electrode and the protein

ASSUNTO(S)

plÃsmons de superfÃcie ciencias exatas e da terra electrochemistry impedance impedÃncia eletroquÃmica concavalina a adsorÃÃo de proteÃna protein adsorption surface plÃsmons concanavalina a

ACESSO AO ARTIGO

Documentos Relacionados