End labeling of enzymatically decapped mRNA.

AUTOR(ES)

Efstratiadis, A

RESUMO

A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.

Documentos Relacionados

• 3'-end-forming signals of yeast mRNA.
• Signals sufficient for 3'-end formation of yeast mRNA.
• Site-specific RNA cleavage generates the 3' end of a poxvirus late mRNA.
• RNase H/oligonucleotide-directed mRNA purification (ROMP) of apoll mRNA.
• Redundant 3' end-forming signals for the yeast CYC1 mRNA.