Endogenous radiolabeling of enterotoxin from Clostridium perfringens type A on a defined medium.
AUTOR(ES)
Granum, P E
RESUMO
Four enterotoxin-positive strains of Clostridium perfringens were tested for sporulation and enterotoxin production on defined media. The medium described by Sacks and Thompson (Appl. Environ. Microbiol. 35:405-409, 1978) gave the highest enterotoxin production and was selected for the production of endogenously labeled enterotoxin. The specific radioactivity of the enterotoxin was 16,000 dpm/microgram when the tritiated amino acids were added to the growth medium just before the inoculum. Addition of the radioactive amino acids during the growth period gave consistently lower specific radioactivity. When the enterotoxin was produced on the medium described by Duncan and Strong (Appl. Microbiol. 16:82-89, 1968), the highest specific radioactivity of the enterotoxin was found when the radioactive amino acids were added to the growth medium 4 h after inoculation. In this case, the specific activity of the enterotoxin was 10,000 dpm/microgram.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=244067Documentos Relacionados
- Enterotoxin formation by Clostridium perfringens type A in a defined medium.
- Characterization of enterotoxin purified from Clostridium perfringens type C.
- Improved method for purification of enterotoxin from Clostridium perfringens type A.
- Biological Characteristics of Clostridium perfringens Type A Enterotoxin
- Clear, defined medium for the sporulation of Clostridium perfringens.
