Enhanced infectivity of adenovirus type 2 DNA and a DNA-protein complex.
AUTOR(ES)
Chinnadurai, G
RESUMO
The infectivity of adenovirus type 2 DNA and a DNA-protein complex was studied in 293 cells, a human embryonic kidney cell line transformed by sheared adenovirus type 5 DNA, and in human KB cells. Adenovirus type 2 DNA was more infectious (up to about 40-fold) in 293 cells than in KB cells, whereas a DNA-protein complex (prepared by a rapid procedure) had about the same infectivity in both cell lines. These data may mean that a factor present in 293 cells (perhaps a viral-coded protein) enhances the infectivity of free viral DNA. The infectivity of DNA and the DNA-protein complex was increased up to fivefold by brief treatment of cell monolayers with 25% dimethyl sulfoxide after transfection. Under these conditions, (i) the infectivity of native adenovirus type 2 DNA ranged from 400 to 1,300 PFU/microgram of DNA in 293 cells and from about 9 to 14 PFU/microgram of DNA in KB cells, and (ii) the infectivity of the DNA-protein complex was 6 X 10(3)to 2 X 10(4) PFU/microgram in 293 cells and 1.4 X 10(4) to 1.6 X 10(4) PFU/microgram in KB cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354049Documentos Relacionados
- Efficient directional cloning of recombinant adenovirus vectors using DNA-protein complex.
- Filter-binding assay for covalent DNA-protein complexes: adenovirus DNA-terminal protein complex.
- Replication of adenovirus DNA-protein complex with purified proteins.
- Transfection of Escherichia coli spheroplasts with a bacteriophage Mu DNA-protein complex.
- Characterization of DNA-protein complexes from simian adenovirus SA7.
