Enhancement of DNA Synthesis in a Mammalian Cell-Free System by Trypsin Treatment

AUTOR(ES)

Brown, Ronald L.

RESUMO

DNA synthesis in a broken cellular preparation of Chinese hamster cells was enhanced approximately 10-fold by a brief trypsin treatment. Alphachymotrypsin also enhanced the synthesis, whereas Pronase did not. The trypsin appears to be acting on a component of the nucleus. Evidence suggests that the trypsin is not removing protein from the DNA, but may be activating the system some other way.

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