Enrichment of Auxotrophic Mutants in Streptomyces griseus
AUTOR(ES)
Werner, Rolf G.
RESUMO
A method for the isolation and enrichment of tryptophan auxotrophic mutants of the indolmycin-producing strain Streptomyces griseus ATCC 12648 was developed, using penicillin selection. With UV irradiation of 2.2 × 104 μW cm−2, a mutation rate to tryptophan auxotrophy of 5.6 × 10−4 was achieved. With 300 μg ml−1 of penicillin G, an enrichment of tryptophan auxotrophs of about 1,000-fold was attained. Approximately 40% of all survivors were tryptophan auxotrophs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=291636Documentos Relacionados
- Enrichment of Auxotrophic Mutants of Aspergillus flavus by Tritium Suicide
- Circularized Chromosome with a Large Palindromic Structure in Streptomyces griseus Mutants
- Purine Auxotrophic Mutants with Altered Spore Color in Streptomyces azureus. ATCC 14921
- Bald mutants of Streptomyces griseus that prematurely undergo key events of sporulation.
- Genetic recombination in Streptomyces griseus.
