Entrapment of plasmid DNA in liposomes.
AUTOR(ES)
Dimitriadis, G J
RESUMO
The entrapment of plasmid DNA (pMB9) and high molecular weight DNA into large unilamellar liposomes is described. The entrapment of DNA is specific and due to encapsulation of DNA into the aqueous compartment of liposomes. The entrapped Dna, resistant to deoxyribonuclease treatment, could be reisolated from liposomes intact and, as has been shown by transformation assay, it remains biologically active. The advantages of our method and possible applications are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327886Documentos Relacionados
- Entrapment of human leukocyte interferon in the aqueous interstices of liposomes.
- Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts.
- Multilamellar structures of DNA complexes with cationic liposomes.
- Proton-hydroxide permeability of liposomes.
- Interaction of immunoglobulins with liposomes.