Enumeration of Activated Thymus-Derived Lymphocytes by the Virus Plaque Assay
AUTOR(ES)
Kano, Shogo
RESUMO
Lymphocytes activated by antigens or mitogens acquire the capacity to replicate viruses, and the number of activated lymphocytes can be estimated by the virus plaque assay. Concanavalin A and pokeweed mitogen produced 33-fold and 17-fold increases in virus plaqueforming cells (V-PFC), respectively, above background, while lipopolysaccharide produced only a 2- to 3-fold increase. T (thymus-derived lymphocyte)-depleted lymphocyte populations, derived from anti-θ-treated or nude (arthymic) mouse spleens, failed to produce V-PFC after culture with concanavalin A or pokeweed mitogen. The present studies thus demonstrate that the virus plaque assay measures activated T-lymphocytes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433722Documentos Relacionados
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