Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.
AUTOR(ES)
Gillam, S
RESUMO
The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393204Documentos Relacionados
- Mutants of Yeast Defective in Iso-1-Cytochrome c
- A Mutator Affecting the Region of the Iso-1-Cytochrome c Gene in Yeast
- Functional characterization of a pyrimidine-rich element in the 5'-noncoding region of the yeast iso-1-cytochrome c gene.
- Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.
- Mapping and Gene Conversion Studies with the Structural Gene for Iso-1-Cytochrome C in Yeast
