ENZYMES IN CANDIDA ALBICANS II. : Tricarboxylic Acid Cycle and Related Enzymes

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Rao, G. Ramananda (Indian Institute of Science, Bangalore, India), M. Sirsi, and T. Ramakrishnan. Enzymes in Candida albicans. II. Tricarboxylic acid cycle and related enzymes. J. Bacteriol. 84:778–783. 1962.—Evidence is presented to show the operation of the tricarboxylic acid cycle in Candida albicans, by studies with whole cells, cell-free preparations, and by the demonstration of most of the enzymes involved in the cycle. Cell-free extracts contained the following enzymes: condensing enzyme; aconitase; isocitric, α-ketoglutaric, succinic, and malic dehydrogenases; malic enzyme; fumarase; reduced diphosphopyridine nucleotide (DPNH) oxidase; DPNH-cytochrome c reductase; reduced triphosphopyridine nucleotide (TPNH) cytochrome c reductase; and diaphorase. Pyruvic dehydrogenase, TPNH oxidase, and transhydrogenase activities could not be detected under the test conditions.

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