Enzymes Related to Lactate Metabolism in Green Algae and Lower Land Plants 1

AUTOR(ES)

Gruber, Peter J.

RESUMO

Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD+-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD+-d-lactate dehydrogenase being soluble. The reduction of pyruvate by NADH proceeded more rapidly than the reverse reaction, and the apparent Michaelis constants for pyruvate and NADH were lower than for d-lactate and NAD+. These data indicated that under physiological conditions, the NAD+-linked d-lactate dehydrogenase probably functions to produce d-lactate from pyruvate.

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