Episomal maintenance of a bovine papilloma virus vector in transgenic mice.
AUTOR(ES)
Elbrecht, A
RESUMO
We have used a bovine papillomavirus-based vector to generate transgenic mice. Transgenic mice result from either pronuclear or cytoplasmic injections of the vector into fertilized eggs. Of 30 mice generated by microinjection, 27 (90%) contained the vector in its episomal form, at less than one copy per cell. This represents a highly efficient means of gene transfer in which the transgene is in a controlled genetic environment.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=365203Documentos Relacionados
- The maintenance of methylation-free islands in transgenic mice.
- Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector.
- Origin of replication in episomal bovine papilloma virus type 1 DNA isolated from transformed cells.
- Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector.
- Retroviral vector sequences inhibit human beta-globin gene expression in transgenic mice.