Epitope Mapping of Neutralizing Botulinum Neurotoxin A Antibodies by Phage Display

AUTOR(ES)

Mullaney, B. P.

FONTE

American Society for Microbiology

RESUMO

Single-chain antibodies neutralize activity and bind nonoverlapping epitopes of botulinum A neurotoxin. Two phage display epitope libraries were constructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selected against the single-chain Fv-Fc antibodies correspond to conformational epitopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), and 889 to 1294 (C25).

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