Escherichia coli mutant which restricts T7 bacteriophage has an altered RNA polymerase.

AUTOR(ES)

Shanblatt, S H

RESUMO

We have previously described an Escherichia coli K-12 mutant, Y49, which restricts the growth of bacteriophage T7 and causes the accumulation of short DNA molecules and head-related particles during infection. We now show that the basis for these effects is the inability of the T7 gene 2 product to inactivate the Y49 RNA polymerase during infection, similar to what has been shown by DeWyngaert and Hinkle (J. Biol. Chem. 254:11247--11253, 1979) for the BR3 and tsnB strains of E. coli.

Documentos Relacionados

• Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase.
• A mutant T7 RNA polymerase as a DNA polymerase.
• The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase.
• I protein: bacteriophage T7-coded inhibitor of Escherichia coli RNA polymerase.
• Cloning and expression of the gene for bacteriophage T7 RNA polymerase.