Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity.
AUTOR(ES)
Mulligan, M E
RESUMO
We describe a simple algorithm for computing a homology score for Escherichia coli promoters based on DNA sequence alone. The homology score was related to 31 values, measured in vitro, of RNA polymerase selectivity, which we define as the product KBk2, the apparent second order rate constant for open complex formation. We found that promoter strength could be predicted to within a factor of +/-4.1 in KBk2 over a range of 10(4) in the same parameter. The quantitative evaluation was linked to an automated (Apple II) procedure for searching and evaluating possible promoters in DNA sequence files.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321093Documentos Relacionados
- Promoter selectivity of Escherichia coli RNA polymerase: effect of base substitutions in the promoter -35 region on promoter strength.
- Promoter selectivity of Escherichia coli RNA polymerase: alteration by fMet-tRNAfMet.
- Promoter selectivity of Escherichia coli RNA polymerase: omega factor is responsible for the ppGpp sensitivity.
- Promoter selectivity of Escherichia coli RNA polymerase sigmaF holoenzyme involved in transcription of flagellar and chemotaxis genes.
- Dye structure affects Taq DNA polymerase terminator selectivity.