Evaluation of a commercial radioassay for the simultaneous estimation of vitamin B12 and folate, with subsequent derivation of the normal reference range.
AUTOR(ES)
Raniolo, E
RESUMO
A commercial assay kit method for the simultaneous estimation of vitamin B12 and folate concentrations has been evaluated. Values derived for folate by a microbiological assay and vitamin B12 by a verified radioassay showed good correlation with the investigated method. The clinical sensitivity of the assay for detecting deficient concentrations of vitamin B12 and folate was comparable to that of the non-commercial methods and other more definitive clinical procedures. Establishment of reference ranges, based on accepted statistical criteria, are discussed and such ranges are contrasted with those proposed by the manufacturer. The kit method is time and labour saving compared with the non-commercial methods.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=499007Documentos Relacionados
- Comparative studies of a new commercial kit for the estimation of vitamin B12 in serum.
- Experience with a commercial kit for the radioisotopic assay of vitamin B12 in serum: the Phadebas B12 Test
- RADIOASSAY OF SERUM VITAMIN B12 BY QUANTITATING THE COMPETITION BETWEEN Co57B12 AND UNLABELED B12 FOR THE BINDING SITES OF INTRINSIC FACTOR*
- Vitamin B12 and folate concentrations in serum and cerebrospinal fluid of neurological patients with special reference to multiple sclerosis and dementia.
- Activities of various cobalamins for Euglena gracilis with reference to vitamin B12 assay with Euglena