Evaluation of a new agar in Uricult-Trio for rapid detection of Escherichia coli in urine.
AUTOR(ES)
Dalet, F
RESUMO
A new commercial agar (Uricult-Trio) with 8-hydroxyquinoline-beta-glucuronide was used to assess 2,536 uropathogens for beta-glucuronidase activity typical of Escherichia coli. Included in the study were 1,807 strains of the family Enterobacteriaceae, 284 strains of nonfermentative bacilli, 345 strains of gram-positive cocci, and 100 yeast strains. In identifying E. coli, the test agar gave a sensitivity of 95.5% and a specificity of 97.2%. Fifty E. coli isolates gave negative reactions; 31 non-E. coli strains produced black colonies characteristic of E. coli. No growth of gram-positive cocci and no false-positive reactions from yeasts were observed. The recovery rate for E. coli on this agar was at least 10% higher than that on blood agar.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=228177Documentos Relacionados
- Development of specific tests for rapid detection of Escherichia coli and all species of Proteus in urine.
- The rapid detection of metronidazole in urine.
- Evaluation of a new tube latex agglutination test for detection of type-specific pneumococcal antigens in urine.
- Rapid detection of Escherichia coli in urine samples by a new chromogenic beta-glucuronidase assay.
- Nutritional requirements for growth of uropathogenic Escherichia coli in human urine.
