Evaluation of a Selective Transport Medium for Gastric Biopsy Specimens To Be Cultured for Helicobacter pylori
AUTOR(ES)
Siu, L. K.
FONTE
American Society for Microbiology
RESUMO
Since the means of culturing Helicobacter pylori may not be available in some laboratories, prolonging the survival of this organism during transportation is a major concern in terms of improving detection rates. A selective transport medium was evaluated for the preservation of H. pylori from 254 gastric biopsy specimens collected from a rural area in China where culturing is not feasible. Gastric biopsy specimens were inoculated in sterile broth consisting of brain heart infusion (BHI) broth, horse serum, and yeast extract supplemented with vancomycin, amphotericin B, and nalidixic acid (VAN). Of the 254 biopsy specimens, 238 were identified by histology to have H. pylori infection. Total rates of recovery of H. pylori from the H. pylori-positive gastric biopsy specimens stored in the BHI-VAN broth ranged from 76 to 46% after storage of specimens for 5 to 9 days. In conclusion, the selective medium is useful for prolonging the survival of H. pylori in gastric biopsy specimens for which immediate culture is not feasible.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=105110Documentos Relacionados
- Effect of transport medium and transportation time on culture of Helicobacter pylori from gastric biopsy specimens.
- Effect of transport medium and transportation time on culture of Helicobacter pylori from gastric biopsy specimens.
- Recovery of Helicobacter pylori from gastric biopsy specimens is not dependent on the transport medium used.
- Transport and Storage of Fresh and Frozen Gastric Biopsy Specimens for Optimal Recovery of Helicobacter pylori
- Evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens of dyspeptic patients
