Evaluation of Detect-A-Strep and the Culturette Ten-Minute Strep ID kits for detection of group A streptococcal antigen in oropharyngeal swabs from children.
AUTOR(ES)
Campos, J M
RESUMO
The Detect-A-Strep kit (DAS) (Antibodies Inc.) and the Culturette Brand 10-Minute Group A Strep ID kit (CBTMSI) (Marion Scientific) were compared with a sensitive culture method for detection of group A streptococci in oropharyngeal swabs from children. Specimens from 953 children ranging in age from 4 months to 18 years were inoculated to selective blood agar plates containing sulfamethoxazole-trimethoprim. Culture plates were incubated anaerobically for the first overnight period and aerobically in the presence of 10% CO2 for the second overnight period. The same specimens were tested within 24 h by DAS (phase 1) or CBTMSI (phase 2). One hundred thirty-five of 538 specimens (25.1%) tested by DAS and 150 of 415 specimens (36.1%) tested by CBTMSI were culture positive. DAS detected 87 of 135 (64.4%) and CBTMSI detected 93 of 150 (62.0%) culture-positive specimens. The specificities of DAS and CBTMSI were 96.5 and 99.6%, respectively. The predictive values of positive and negative results were 86.1 and 89.0% for DAS and 98.9 and 82.2% for CBTMSI. Because a reliable distinction between infected patients and carrier state patients cannot be made, we conclude that neither DAS nor CBTMSI is sufficiently sensitive to replace our culture method.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=268347Documentos Relacionados
- Evaluation of the Culturette Brand Ten-Minute Group A Strep ID technique.
- Detection of group A streptococcal antigen directly from throat swabs with a ten-minute latex agglutination test.
- Ten-minute detection of group A streptococci in pediatric throat swabs.
- Ten-Minute Test for Differentiating Between Klebsiella and Enterobacter Isolates
- Evaluation of a rapid method for the detection of streptococcal group A antigen directly from throat swabs.