Evaluation of Enzyme-Linked Immunosorbent Assays and a PCR Test for Detection of Shiga Toxins for Shiga Toxin-Producing Escherichia coli in Cattle Herds
AUTOR(ES)
Segura-Alvarez, Monserrat
FONTE
American Society for Microbiology
RESUMO
Antigen capture enzyme-linked immunosorbent assays (ELISAs) for the detection of Stx1 and/or Stx2 in cattle feces were validated in comparison to the Vero cell cytotoxicity neutralization test (as a “gold standard”) applied in the course of a monitoring program for Shiga toxin-producing Escherichia coli in German cattle herds as a prescreening test and compared to MK1/MK2 PCR as an alternative prescreening test.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=308972Documentos Relacionados
- Comparison of a Shiga Toxin Enzyme-Linked Immunosorbent Assay and Two Types of PCR for Detection of Shiga Toxin-Producing Escherichia coli in Human Stool Specimens
- Double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of toxin-producing Corynebacterium diphtheriae.
- Serodiagnosis by passive hemagglutination test and verotoxin enzyme-linked immunosorbent assay of toxin-producing Escherichia coli infections in patients with hemolytic-uremic syndrome.
- Development and evaluation of enzyme-linked immunosorbent assays for detection of shiga-like toxin I and shiga-like toxin II.
- Application of an enzyme-linked immunosorbent assay for screening of T-2 toxin-producing Fusarium spp.
