Evaluation of the accuracy of various phenotypic tests to detect oxacillin resistance in coagulase-negative staphylococci
AUTOR(ES)
Perez, Leandro Reus Rodrigues, d'Azevedo, Pedro Alves
FONTE
Brazilian Journal of Infectious Diseases
DATA DE PUBLICAÇÃO
2008-06
RESUMO
In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30µg-cefoxitin and 1µg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. Of these, 138 (78.4%) harbored the mecA gene. Using 30µg-cefoxitin and 1µg-oxacillin disks we obtained 100 and 98.3% accuracy, respectively. In addition, when cefoxitin was used as marker in an agar dilution method, the higher accuracy (99.4%) was established with 8mg cefoxitin per mL breakpoint. Thus, despite of the agar dilution method using cefoxitin as a marker not being standard for this detection, our results suggested that it is an excellent alternative to detect the oxacillin resistance/mecA gene among CoNS isolates.
Documentos Relacionados
- Coagulase-Negative Staphylococci: Comparison of Phenotypic and Genotypic Oxacillin Susceptibility Tests and Evaluation of the Agar Screening Test by Using Different Concentrations of Oxacillin
- Coagulase-Negative Staphylococci: Comparison of Phenotypic and Genotypic Oxacillin Susceptibility Tests and Evaluation of the Agar Screening Test by Using Different Concentrations of Oxacillin
- Evaluation of a Latex Agglutination Test (MRSA-Screen) for Detection of Oxacillin Resistance in Coagulase-Negative Staphylococci
- Ability of the Modified Vitek Card To Detect Coagulase-Negative Staphylococci with mecA and Oxacillin-Resistant Phenotypes
- Coagulase-negative staphylococci strains resistant to oxacillin isolated from neonatal blood cultures