Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-Positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens

AUTOR(ES)
FONTE

American Society for Microbiology

RESUMO

The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in diluted phosphate-buffered saline, respectively.

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