EVIDENCE FOR MULTIPLE GENE CONTROL OF A SINGLE POLYPEPTIDE CHAIN: THE HEAVY CHAIN OF RABBIT IMMUNOGLOBULIN

AUTOR(ES)

Koshland, Marian Elliott

RESUMO

To determine the chemical basis for rabbit heavy chain allotypes, amino acid analyses were carried out on IgG and IgM antibodies isolated from rabbits which were homozygous a1 or a3 for the γ chain locus and homozygous b4 for the light chain locus. The compositional differences between a1 and a3 IgM antibodies were found to be identical to those between their IgG counterparts. The identity of the allotypic amino acid replacements showed that the same genetic markers were present in the variable sequences of IgG and IgM heavy chains. Since the constant sequences of these heavy chains are controlled by different loci, these data demonstrated that rabbit heavy chains are coded by two separate germ-line genes, one producing the variable region and a second one of various genes producing the constant regions.

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