Evidence for translocation of DNA sequences during sea urchin embryogenesis.

AUTOR(ES)

Dickinson, D G

RESUMO

Hairpin-like DNA was prepared in vitro from the family of sequences that are inverted relative to each other and, as pairs, are relatively homologous and adjacent on the sea urchin genome. The majority of these hairpins are shown to have base pair mismatch positions distributed along their stems. Comparison of the hairpins derived from the DNA of morula, blastula, and gastrula stage embryos shows that during embryogenesis there are changes in the average number and position of S1 nuclease-sensitive base pair mismatch sites on the majority of the hairpin stems. Our data indicate that during early embryogenesis there are sequence changes in vivo within the majority of the adjacent inverted repeat sequences of the sea urchin genome. We have also found that there is higher specificity for the occurrence of sequence-change events within that fraction of the inverted repeat sequences that are methylated in vivo.

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