Evidences of two different sets of histone genes active during embryogenesis of the sea urchin Paracentrotus lividus.
AUTOR(ES)
Spinelli, G
RESUMO
Histone mRNAs at different stages of development were purified by hybridization with the cloned homologous histone genes. The electrophoretic patterns of oocytes, 2-4 blastomeres, 64 cells and morula histone mRNAs was found to be identical, whereas the electrophoretic pattern of mesenchyme blastula histone mRNA was markedly different. The cloned histone DNA of P.lividus was hybridized with the RNA of each stage. The Tm was 74 degrees C in all cases except for the mesenchyme histone mRNAs whose Tm was 59 degrees C, thus suggesting that at least two different clusters of histone genes are active in the course of the sea urchin development.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327712Documentos Relacionados
- Aminoacyl-sRNA synthetases as possible regulators of protein synthesis in the embryo of the sea urchin Paracentrotus lividus.
- Nucleotide sequence of a full length cDNA clone encoding for beta-tubulin of the sea urchin Paracentrotus lividus.
- Transient alterations of the chromatin structure of sea urchin early histone genes during embryogenesis.
- Spatial distribution of two maternal messengers in Paracentrotus lividus during oogenesis and embryogenesis.
- Identification of two homologous mitochondrial DNA sequences, which bind strongly and specifically to a mitochondrial protein of Paracentrotus lividus.