Evolution of a Lytic Bacteriophage via DNA Acquisition from the Lactococcus lactis Chromosome †
AUTOR(ES)
Moineau, Sylvain
RESUMO
We discovered a phage-host interaction in which the lytic phage ul36, in response to pressure exerted by an abortive phage resistance mechanism, acquired a large DNA fragment from the chromosome of Lactococcus lactis NCK203 to form a new phage, ul37. Phage ul37 was characterized at morphological, phenotypic, and genotypic levels and was found to be a member of the P335 species. Although it exhibits a high level of DNA homology with ul36, phage ul37 is resistant to the abortive mechanism and has a longer tail, a different base plate, and apparently a different origin of replication. The chromosomal DNA implicated in the formation of new phage ul37 was disrupted by site-specific integration in NCK203. This strategy prevented the appearance of ul37 during subsequent infections with ul36.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201570Documentos Relacionados
- An origin of DNA replication from Lactococcus lactis bacteriophage c2.
- Gene Cloning and Expression and Secretion of Listeria monocytogenes Bacteriophage-Lytic Enzymes in Lactococcus lactis
- Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis.
- Bacteriophage Resistance of a ΔthyA Mutant of Lactococcus lactis Blocked in DNA Replication
- Controlled Integration into the Lactococcus Chromosome of the pCI829-Encoded Abortive Infection Gene from Lactococcus lactis subsp. lactis UC811