Ex Vivo Phenotype and Frequency of Influenza Virus-Specific CD4 Memory T Cells

AUTOR(ES)

Lucas, Michaela

FONTE

American Society for Microbiology

RESUMO

Recent advances in class II tetramer staining technology have allowed reliable direct ex vivo visualization of antigen-specific CD4 T cells. In order to define the frequency and phenotype of a prototype response to a nonpersistent pathogen, we have used such techniques to analyze influenza virus-specific memory CD4 T cells directly from blood. These responses are stably detectable ex vivo at low frequencies (range, 0.00012 to 0.0061% of CD4 T cells) and display a distinct “central memory” CD62L+ phenotype.

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