Examination of Methods for Enumerating Hemicellulose-Utilizing Bacteria in the Rumen
AUTOR(ES)
Henning, Penelope A.
RESUMO
Counts of colonies that developed after 4 days on agar medium containing 0.3% xylan and preincubated rumen fluid were similar to counts of xylanolytic bacteria obtained when total culturable counts were multiplied by the percentage of isolates capable of producing acid from xylan. Shortening the incubation period reduced the chance of including satellite colonies of non-xylanolytic organisms in the count. Nearly all of the xylanolytic isolates irrespective of the medium from which they were isolated degraded and utilized xylan extensively. The use of a culture medium containing a high concentration (3%) of xylan is also described. The number of colonies capable of producing clearings in this medium was less than 10% of the total culturable counts. Isolates from such colonies were shown to produce diffusible (extracellular) xylanases.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=243427Documentos Relacionados
- Comparison of Methods for Enumerating Fluorescent Bacteria1
- Basal medium for the selective enumeration of rumen bacteria utilizing specific energy sources.
- Characteristics of Saponin-Utilizing Bacteria from the Rumen of Cattle
- Mechanism of Isolated Hemicellulose and Xylan Degradation by Cellulolytic Rumen Bacteria1
- Enumeration and Isolation of Lactate-Utilizing Bacteria from the Rumen of Sheep