Experience with an enzyme immunoassay for serotyping human group A rotaviruses.
AUTOR(ES)
Unicomb, L E
RESUMO
An enzyme immunoassay utilizing neutralizing monoclonal antibodies to VP7 of four human group A rotavirus serotypes successfully typed rotaviruses in 71.4% (568 of 796) of fecal specimens. Sensitivity was enhanced by using homologous capture and detector antibodies. Serotyping was most successful with specimens stored for less than 3 years and containing 10(4) or more particles per ml.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=267369Documentos Relacionados
- Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.
- Reactivities of serotyping monoclonal antibodies with culture-adapted human rotaviruses.
- Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses.
- Evidence of serologic diversity within group C rotaviruses.
- Development of a biotin-streptavidin-enhanced enzyme-linked immunosorbent assay which uses monoclonal antibodies for detection of group C rotaviruses.