Export of alpha-amylase by Bacillus amyloliquefaciens requires proton motive force.
AUTOR(ES)
Murén, E M
RESUMO
The secretion of protein directly into the extracellular medium by Bacillus amyloliquefaciens, a gram-positive bacterium, was shown to be dependent on proton motive force. When the electrochemical membrane potential gradient of protons was dissipated either by uncouplers or by valinomycin in combination with K+, a precursor form of alpha-amylase accumulated on the cellular membrane. The proton motive force could be dissipated without altering the intracellular level of ATP, indicating that the observed inhibition of export was not the result of decreased ATP concentration.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214310Documentos Relacionados
- Increased production of alpha-amylase by Bacillus amyloliquefaciens in the presence of glycine.
- alpha-amylase from five strains of Bacillus amyloliquefaciens: evidence for identical primary structures.
- Expression of alpha-amylase in Bacillus licheniformis.
- Complete nucleotide sequence of an alpha-amylase gene from Bacillus subtilis 2633, an alpha-amylase extrahyperproducing strain.
- Immobilization of alpha-amylase produced by Bacillus circulans GRS 313