Expressão e purificação da proteína MaSp 2 isolada da glândula produtora de teia da aranha parawixia bistriata

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Spider silks are constituted essentially of proteins, secreted by specialized glands found in different groups of arthropods. All spider species can produce silks, biopolymers synthesized by abdominal glands and polymerized through a series of spinnerets. Spiders may present seven different types of glands, each producing different fibers. These differences between fibers allow the comparison of the structure of the proteins with its function, since each one presents distinct mechanical properties being composed by diverse repetitive modules that confer these properties. Spider silks have demonstrated superior mechanical properties involving fiber resistance when compared to silk produced by Bombyx mori. The possibility of producing new biomaterials from spider silk (spidroin) with similar properties motivated this research. Despite their superior mechanical characteristics, the inability to domesticate the spiders to produce enough protein for industrial applications had lead to the development of alternative strategies for silk production, mainly the recombinant DNA technology. This project involves the recombinant expression of spider biopolymers in microorganisms through the manipulation of silk genes isolated from the major ampullate gland of the Brazilian species Parawixia bistriata.

ASSUNTO(S)

parawixia bistriata major ampullate gland ciências agrárias parawixia bistriata biopolymers recombinant dna biopolímeros, glândula ampolada principal seda spider silk

ACESSO AO ARTIGO

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