Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells.
AUTOR(ES)
O'Reilly, D R
RESUMO
Recombinant baculoviruses were constructed which express simian virus 40 large T antigen (SVT-Ag) or murine p53 to high levels in infected insect cells. Characterization of the expressed proteins revealed that they display many properties of the corresponding mammalian-derived proteins. Both proteins are of wild-type size, localize to the nucleus, are recognized by several SVT-Ag- or p53-specific monoclonal antibodies, and are phosphorylated in this system. Complexes are formed between baculovirus-derived SVT-Ag and p53 after coinfection of insect cells with both recombinant viruses. After infection of insect cells with either virus individually, each protein can self-associate to form a variety of oligomeric species. Pulse-chase experiments indicated that both SVT-Ag and p53 are highly stable in insect cells, even in the absence of complex formation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=253427Documentos Relacionados
- Identification of the p53 protein domain involved in formation of the simian virus 40 large T-antigen-p53 protein complex.
- Complex formation of simian virus 40 large T antigen with cellular protein p53.
- The p53 complex from monkey cells modulates the biochemical activities of simian virus 40 large T antigen.
- Intracellular Location and Kinetics of Complex Formation Between Simian Virus 40 T Antigen and Cellular Protein p53
- p53 Targets Simian Virus 40 Large T Antigen for Acetylation by CBP