Expression and rearrangement of homologous immunoglobulin VH genes in two mouse strains.
AUTOR(ES)
Near, R I
RESUMO
A family of murine anti-p-azophenylarsonate (Ars) antibodies share a variable (V) region serologically defined marker, the 36-60 idiotype (Id36-60). Most mouse strains possess five genes highly homologous to the gene encoding the heavy (H) chain V region of antibodies bearing Id36-60 (VH36-60); however, only one of these genes is ever utilized by hybridomas whose antibodies bind Ars and bear Id36-60. The relevant VH genes were cloned from A/J and BALB/c mouse DNA libraries. Their DNA sequences were found to differ at only two positions. Southern blot analysis, protein sequence determination, and nucleic acid sequence determination indicate that the above hybridomas utilize the same joining (JH3), diversity (D), and VH gene segments regardless of BALB/c or A/J strain origin. Despite this virtual identity, BALB/c and A/J mouse strains express quite different serum levels of Id36-60-bearing antibodies when immunized with Ars. The basis of this regulatory process is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=345458Documentos Relacionados
- Allelic immunoglobulin VH genes in two mouse strains: possible germline gene recombination.
- Organization of the murine immunoglobulin VH complex in the inbred strains.
- Structural relationships among mouse and human immunoglobulin VH genes in the subgroup III.
- Rearrangement and expression of erythropoietin genes in transformed mouse cells.
- Rearrangement and expression of immunoglobulin genes and expression of Tac antigen in hairy cell leukemia.