Expression of a complete soybean leghemoglobin gene in root nodules of transgenic Lotus corniculatus

AUTOR(ES)

Stougaard, Jens

RESUMO

The complete soybean leghemoglobin lbc3 gene was transferred into the legume Lotus corniculatus using an Agrobacterium rhizogenes vector system. Organ-specific expression of the soybean gene was observed in root nodules formed on regenerated transgenic plants after infection with Rhizobium loti. The primary transcript was processed in the same way as in soybean nodules and the resulting mRNA was translated into Lbc3 protein. Quantitative determination of the Lbc3 protein in nodules of transgenic plants indicated that the steady-state level of the soybean protein is comparable to that of endogenous Lotus leghemoglobin.

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