Expression of a Thiobacillus ferrooxidans origin of replication in Escherichia coli.
AUTOR(ES)
Rawlings, D E
RESUMO
A cryptic plasmid from an autotrophically grown arsenic-resistant strain of Thiobacillus ferrooxidans was isolated and cloned into pBR325. The origin of replication of pBR325 was deleted, and the recombinant plasmid was shown to replicate in Escherichia coli, using an origin of replication located on the Thiobacillus plasmid.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=215493Documentos Relacionados
- Cloning of a Thiobacillus ferrooxidans plasmid in Escherichia coli.
- Cloning and expression of Thiobacillus ferrooxidans mercury ion resistance genes in Escherichia coli.
- Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli.
- Thiobacillus ferrooxidans tyrosyl-tRNA synthetase functions in vivo in Escherichia coli.
- Isolation of a replication origin complex from Escherichia coli.